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Old 10-12-2010, 03:41 AM   #1
barrmur
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Location: Nottingham, England

Join Date: Nov 2009
Posts: 26
Default Sequencing small DNA fragments (25bp)

Hi all,

Just wondering if anyone has any experience of the following. I want to sequence a single fragment of RNA to confirm it is what I think it is. Any suggestions on how to go about it. The major problme is it is only 25 bases long. I can convert to DNA and have a 7bp primer but not sure where to go from here. I have access to illumina, pyromark Q24, ABI 3730. I have tried cloning the fragment with no success. Any other suggestions.
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