Hi everyone,
We would like to test population effect on DE (face to a toxicant) using mRNAseq. We need samples to be representative of each population.
Is it correct to pool different individuals to construct a library, and to consider libraries made from different sets of individuals as biological replicates at the population level ? (e.g. 1+2+3 = R1; 4+5+6 = R2). Do you know exemples where such libraries have been used ?
Thanks in advance for any advice
We would like to test population effect on DE (face to a toxicant) using mRNAseq. We need samples to be representative of each population.
Is it correct to pool different individuals to construct a library, and to consider libraries made from different sets of individuals as biological replicates at the population level ? (e.g. 1+2+3 = R1; 4+5+6 = R2). Do you know exemples where such libraries have been used ?
Thanks in advance for any advice
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