Methylation in the + strand influence the expression of gene in the - strand?

yao_licr · 09-19-2014, 06:35 AM

Hi all,
I have a general question about how the methylation within the TSS 1000 bp will influence the expression of the gene?

It is easy to understand that if the methylation happens in the same strand as the gene sits, it will potentially repress the expression of the corresponding gene; but if the methylation (within TSS 1000 bp) happens in the + strand, so could it potentially repress the corresponding gene in the - strand as well? Is there any literature about that?

Thanks a lot!

Yao

dpryan · 09-19-2014, 07:34 AM

It's highly likely that methylation on the + strand can affect transcription on the - strand. I've never read a paper that showed that, but I've also never looked.

volks · 09-27-2014, 10:18 PM

most CpGs in the genome are either unmethylated or methylated on both strands (CG is a palindromic sequence). any hemi-methylated CpGs are quickly methylated on both strands by DNMT1 (that is what is happening for every cellular replication cycle). i am not aware of any finding that would answer your question more appropriately.

hamarillo · 10-22-2015, 11:31 AM

Bottom line is, how could enzymes distinguish which strand they are methylating?

when you perform a sequencing experiment and on the analysis you find that there is no equilibrium between the methylation at - and + strands; is it an indicator of an experimental artefact or a sequencer failure?

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09-19-2014, 06:35 AM	#1
yao_licr Junior Member Location: sweden Join Date: Jul 2014 Posts: 7	Methylation in the + strand influence the expression of gene in the - strand? Hi all, I have a general question about how the methylation within the TSS 1000 bp will influence the expression of the gene? It is easy to understand that if the methylation happens in the same strand as the gene sits, it will potentially repress the expression of the corresponding gene; but if the methylation (within TSS 1000 bp) happens in the + strand, so could it potentially repress the corresponding gene in the - strand as well? Is there any literature about that? Thanks a lot! Yao

09-19-2014, 07:34 AM	#2
dpryan Devon Ryan Location: Freiburg, Germany Join Date: Jul 2011 Posts: 3,478	It's highly likely that methylation on the + strand can affect transcription on the - strand. I've never read a paper that showed that, but I've also never looked.

09-27-2014, 10:18 PM	#3
volks Member Location: hd.de Join Date: Jun 2010 Posts: 81	most CpGs in the genome are either unmethylated or methylated on both strands (CG is a palindromic sequence). any hemi-methylated CpGs are quickly methylated on both strands by DNMT1 (that is what is happening for every cellular replication cycle). i am not aware of any finding that would answer your question more appropriately.

10-22-2015, 11:31 AM	#4
hamarillo Junior Member Location: Mexico Join Date: Aug 2012 Posts: 1	Bottom line is, how could enzymes distinguish which strand they are methylating? when you perform a sequencing experiment and on the analysis you find that there is no equilibrium between the methylation at - and + strands; is it an indicator of an experimental artefact or a sequencer failure?