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Thread | Thread Starter | Forum | Replies | Last Post |
Does Strand-specific RNA-seq mean reads from one genome strand? | shangzhong0619 | RNA Sequencing | 4 | 06-16-2014 12:56 AM |
Strand bias on estimation of DNA methylation level | hui_shi | Bioinformatics | 4 | 04-23-2014 12:57 PM |
how to know a gene's strand | ahli1981 | Bioinformatics | 1 | 01-30-2012 07:44 AM |
always reads on plus strand more than on minus strand | tujchl | Bioinformatics | 4 | 04-29-2011 12:08 AM |
different number of reads mapped to plus strand and minus strand | gfmgfm | Bioinformatics | 2 | 02-03-2011 10:26 AM |
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#1 |
Junior Member
Location: sweden Join Date: Jul 2014
Posts: 7
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Hi all,
I have a general question about how the methylation within the TSS 1000 bp will influence the expression of the gene? It is easy to understand that if the methylation happens in the same strand as the gene sits, it will potentially repress the expression of the corresponding gene; but if the methylation (within TSS 1000 bp) happens in the + strand, so could it potentially repress the corresponding gene in the - strand as well? Is there any literature about that? Thanks a lot! Yao |
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#2 |
Devon Ryan
Location: Freiburg, Germany Join Date: Jul 2011
Posts: 3,478
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It's highly likely that methylation on the + strand can affect transcription on the - strand. I've never read a paper that showed that, but I've also never looked.
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#3 |
Member
Location: hd.de Join Date: Jun 2010
Posts: 81
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most CpGs in the genome are either unmethylated or methylated on both strands (CG is a palindromic sequence). any hemi-methylated CpGs are quickly methylated on both strands by DNMT1 (that is what is happening for every cellular replication cycle). i am not aware of any finding that would answer your question more appropriately.
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#4 |
Junior Member
Location: Mexico Join Date: Aug 2012
Posts: 1
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Bottom line is, how could enzymes distinguish which strand they are methylating?
when you perform a sequencing experiment and on the analysis you find that there is no equilibrium between the methylation at - and + strands; is it an indicator of an experimental artefact or a sequencer failure? |
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