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Old 03-07-2016, 09:20 PM   #1
aquleaf
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Location: Baltimore

Join Date: Mar 2010
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Default Insert size range in ChIP-Seq

Hi All. May I ask a question regarding ChIP-Seq library preparation?

The input looks good with summit at ~200bp according to Bioanalyzer. However, the size distribution of ChIPed DNA shift with summit at ~900bp. It seems that 100-500bp is usually selected to make a library. Is that OK if we select 100bp-1kb to make a library? What is the drawback if we increase the range? Thanks a bunch! Any suggestion will be greatly appreciated.
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