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  • Controversy over RNA-DNA Differences findings...

    There is a pretty interesting controversy surrounding the "groundbreaking" findings of widespread differences between RNA and the DNA from which it was transcribed.

    Genomes Unzipped has done a great job of summarizing the rebuttals to the original paper...regardless of what the final verdict is, the arguments highlight some of the major artefacts that can sneak into sequencing data conclusions:
    1. Sample prep artefacts
    2. Failure to trim reads
    3. Mismapping due to paralogs
    4. Mapping to incomplete references

Latest Articles

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  • seqadmin
    Strategies for Sequencing Challenging Samples
    by seqadmin


    Despite advancements in sequencing platforms and related sample preparation technologies, certain sample types continue to present significant challenges that can compromise sequencing results. Pedro Echave, Senior Manager of the Global Business Segment at Revvity, explained that the success of a sequencing experiment ultimately depends on the amount and integrity of the nucleic acid template (RNA or DNA) obtained from a sample. “The better the quality of the nucleic acid isolated...
    03-22-2024, 06:39 AM
  • seqadmin
    Techniques and Challenges in Conservation Genomics
    by seqadmin



    The field of conservation genomics centers on applying genomics technologies in support of conservation efforts and the preservation of biodiversity. This article features interviews with two researchers who showcase their innovative work and highlight the current state and future of conservation genomics.

    Avian Conservation
    Matthew DeSaix, a recent doctoral graduate from Kristen Ruegg’s lab at The University of Colorado, shared that most of his research...
    03-08-2024, 10:41 AM

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