We are using the QSONICA Q800R machine for a while, in order to have fragmented gDNA at the size of 250-300bp. After sonication we tried Thermo Scientific PCR cleanup kit, and then applied the NEBNext DNA library prep kit for Illumina. After size selection of adaptor – ligated DNA, there are still unwanted large fragments.
Do you have any ideas to remove this unwanted lenghts?
Thanks in advance,
Adi
Do you have any ideas to remove this unwanted lenghts?
Thanks in advance,
Adi
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