Hi to all. Unfortunately I still without understand completely the NGS technology. So, I need that someone give me some tips or workflow to carry out succesfully this experiment in a Miseq machine:
I want to sequence the 5S rDNA (~1500bp), the 18S rDNA (~1700bp) and the mitochondrial genome of 140 individuals of elasmobranchs (sharks and rays), so I will execute more than 420 sequencing. The mitochondrial genome will be amplified by the Multiple Displacement Amplification technique (http://en.wikipedia.org/wiki/Multipl..._amplification).
I read that I need the Nextera XT kit and the guide seems to be easy, but I not understand yet (if is neccesary) how to use the dual indexing to sequence my samples.
I appreciate any comments to help me clarify this problem.
I want to sequence the 5S rDNA (~1500bp), the 18S rDNA (~1700bp) and the mitochondrial genome of 140 individuals of elasmobranchs (sharks and rays), so I will execute more than 420 sequencing. The mitochondrial genome will be amplified by the Multiple Displacement Amplification technique (http://en.wikipedia.org/wiki/Multipl..._amplification).
I read that I need the Nextera XT kit and the guide seems to be easy, but I not understand yet (if is neccesary) how to use the dual indexing to sequence my samples.
I appreciate any comments to help me clarify this problem.
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