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Old 06-28-2010, 06:56 AM   #1
Location: USA

Join Date: Mar 2010
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Default strand-specific Illumina protocol

Is there any way to tell from the sequence data whether an RNA-Seq Illumina run was done on strand specific data or not?

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Old 06-28-2010, 07:53 AM   #2
Lee Sam
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I would assume that you could tell by looking at the reads aligning to the genes. If there's a mix of forward and reverse aligning reads, I might assume that the library was not prepped using a strand-specific protocol.
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