Tweet
We ran mRNA instead of total RNA assays, and are left with rRNA contamination values instead of RINs. The traces of the RNAs all look fine, so it seems that the data are there, and all we need is to find a way to get the RIN from them. Is there a way to (non-manually) obtain RINs after running mRNA assays? I also posted this question as a response to a related thread in this forum, but decided to create a new thread in addition, because it seems like a problem that is not unlikely to occur to people doing library preps. Thanks for any help.
-
-
No, I don't think this is possible. But if you figure out a way, please post it.
--
Phillip -
I recall reading that RIN is the value assigned mainly based on rRNA peaks. So even if you'd get RIN value for your samples it might be wrong because without rRNA peaks the algorithm might misinterpret the RNA integrity. This is how I understood it.Comment
-
The field of epigenetics has traditionally concentrated more on DNA and how changes like methylation and phosphorylation of histones impact gene expression and regulation. However, our increased understanding of RNA modifications and their importance in cellular processes has led to a rise in epitranscriptomics research. “Epitranscriptomics brings together the concepts of epigenetics and gene expression,” explained Adrien Leger, PhD, Principal Research Scientist...04-22-2024, 07:01 AM -
Proteins are often described as the workhorses of the cell, and identifying their sequences is key to understanding their role in biological processes and disease. Currently, the most common technique used to determine protein sequences is mass spectrometry. While still a valuable tool, mass spectrometry faces several limitations and requires a highly experienced scientist familiar with the equipment to operate it. Additionally, other proteomic methods, like affinity assays, are constrained...04-04-2024, 04:25 PM
Comment