Originally posted by nahmed
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Yes, HiSeq 2500 in High Output mode (v4) yields ~1TB of data in 6 days. This is due to capturing the data for each lane and surface in 3 passes instead of 2 like the HiSeq 4000. The HiSeq 2500's capable of running the v4 chemistry actually are scanning a little faster than the HiSeq 4000. That third pass to image each lane & surface (and the re-trace step) add up to about a 30 minute cycle time on HiSeq 2500. The chemistry isn't older at all, just an Illumina imposition to the supported read length.
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