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Old 03-22-2010, 05:53 AM   #1
severin
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Default FRT-seq: amplification-free, strand-specific transcriptome sequencing

Brief Communication

Nature Methods 7, 130 - 132 (2010)
Published online: 17 January 2010 | doi:10.1038/nmeth.1417

FRT-seq: amplification-free, strand-specific transcriptome sequencing

Lira Mamanova1,3, Robert M Andrews1,3, Keith D James1, Elizabeth M Sheridan1, Peter D Ellis1, Cordelia F Langford1, Tobias W B Ost2, John E Collins1 & Daniel J Turner1

Abstract

We report an alternative approach to transcriptome sequencing for the Illumina Genome Analyzer, in which the reverse transcription reaction takes place on the flowcell. No amplification is performed during the library preparation, so PCR biases and duplicates are avoided, and because the template is poly(A)+ RNA rather than cDNA, the resulting sequences are necessarily strand-specific. The method is compatible with paired- or single-end sequencing.
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