Hello,
I am looking to use mpileup for base calling. Previously I ran samples together but could not find any sample specific read count/quality information. My sample format was PLP:SP. I am only expecting one of my samples to contain a SNP. I would like for each sample info to contain ref/non-ref read counts as well as the qualities associated with them.
Ideally I would like to have the data found in the I16 info for each sample.
What arguments would I need to run mpileup with? Do I have to run mpileup on each sample separately?
Thanks!
I am looking to use mpileup for base calling. Previously I ran samples together but could not find any sample specific read count/quality information. My sample format was PLP:SP. I am only expecting one of my samples to contain a SNP. I would like for each sample info to contain ref/non-ref read counts as well as the qualities associated with them.
Ideally I would like to have the data found in the I16 info for each sample.
What arguments would I need to run mpileup with? Do I have to run mpileup on each sample separately?
Thanks!