I am using ScriptSeq Complete Kit (Bacteria) to prepare RNA-Seq library. I finished rRNA depletion step and I checked RNA concentration with Nanodrop. I found I might lose RNA from several samples. There RNA concentrations are very low with incorrect 260/280 compared with the other samples.
I think the RNA might be lost in the step of ethanol precipitation and washing. I want to reprocess these samples. I calculate the remaining reagents and I found I have enough reagents except for the magnetic beads. The kit containing is just too expensive. Anything else I can do? Thank you!
I think the RNA might be lost in the step of ethanol precipitation and washing. I want to reprocess these samples. I calculate the remaining reagents and I found I have enough reagents except for the magnetic beads. The kit containing is just too expensive. Anything else I can do? Thank you!
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