I have two tubes of a precious, irreplaceable sample with approximately 20 cells of an unknown eukaryotic microbe and would like to get a transcriptome out of it. As far as I could find out, the way to go is spinning down the cells and proceed with a typical RNA prep.
I have two important questions before proceeding: Is there any other recommendation for a minute sample stored in RNAlater?
What is safer: do RNA amplification and then cDNA or do cDNA and then DNA amplification?
Thanks,
I have two important questions before proceeding: Is there any other recommendation for a minute sample stored in RNAlater?
What is safer: do RNA amplification and then cDNA or do cDNA and then DNA amplification?
Thanks,
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