My RNA-seq data is non-strand specific.
After mapping data to genome by bowTie,
I confused meaning in results file : strand '+' or '-' ?
for example:
readscore1 + chr1 10000 tag1 ...
readscore2 - chr1 10000 tag2 ...
Does it mean that the positions as above are in the same place of genome
and they are complementation?
Is it related to transcription direction?
After mapping data to genome by bowTie,
I confused meaning in results file : strand '+' or '-' ?
for example:
readscore1 + chr1 10000 tag1 ...
readscore2 - chr1 10000 tag2 ...
Does it mean that the positions as above are in the same place of genome
and they are complementation?
Is it related to transcription direction?
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