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Old 07-06-2016, 05:26 AM   #1
Pab77
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Location: Seattle

Join Date: Feb 2012
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Default Ion PI v2 chip - prepared chips stored for over 24 hours

Dear all,
Has anyone tried re-washing prepared Ion PI chips after prepping and storing them for >24-48 hrs? Our FAS told us that it's ok to re-wash once after such storage and we have used chips like that without any problems. But now we are in the predicament of having to wait another day for such a chip because our templating is too high to do the sequencing today. We used the OT2 and ended up with 39% of templated ISPs. I'm thinking it should be fine to wash this chip one more time and use tomorrow but any thought would be much appreciated.
Thank you!
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