SEQanswers

Go Back   SEQanswers > Sequencing Technologies/Companies > Ion Torrent



Similar Threads
Thread Thread Starter Forum Replies Last Post
ddRAD library low concentration dBlatt Sample Prep / Library Generation 3 07-03-2017 11:30 AM
Library prep/low concentration Normal Sample Prep / Library Generation 1 03-19-2015 03:12 PM
Wang, 'Low Cost Library Construction...': Low Concentration MAdkisson RNA Sequencing 2 02-19-2014 02:33 PM
low library concentration biochembug Illumina/Solexa 0 08-02-2012 06:13 AM
Low concentration GA library Melanie Sample Prep / Library Generation 4 08-04-2010 01:34 PM

Reply
 
Thread Tools
Old 04-14-2017, 12:31 AM   #1
hanco
Junior Member
 
Location: Japan

Join Date: Jun 2015
Posts: 8
Default Low Concentration Library

Hi,

I made libraries using Colon and Lung Cancer Research Panel and quantify them using qPCR. I was wondering why some of the libraries have less concentration than the others. I made libraries from serum DNA, the starting material is 50 ng and made all the libraries at the same time.
Can someone help me with the answer? I really appreciate it.

Thank you!
hanco is offline   Reply With Quote
Old 04-14-2017, 08:34 AM   #2
pmiguel
Senior Member
 
Location: Purdue University, West Lafayette, Indiana

Join Date: Aug 2008
Posts: 2,236
Default

Hi Hanco,
There is no single answer to your question. Basically every step in your protocol has the potential to cause library construction to occur sub-optimally or even fail altogether. Also many methods of quantitating the amount of input material will be inaccurate. So the actual amount of material used for library construction may be highly variable.

--
Phillip
pmiguel is offline   Reply With Quote
Old 04-16-2017, 04:53 PM   #3
hanco
Junior Member
 
Location: Japan

Join Date: Jun 2015
Posts: 8
Default

Hi Philllip,

I thought perhaps the quality of DNA that I used to make the libraries already damaged from the beginning or my technical error.
Anyway, thank you so much for your answer.

Hanco
hanco is offline   Reply With Quote
Reply

Tags
ion torrent, library, library concentration, qpcr, real time pcr

Thread Tools

Posting Rules
You may not post new threads
You may not post replies
You may not post attachments
You may not edit your posts

BB code is On
Smilies are On
[IMG] code is On
HTML code is Off




All times are GMT -8. The time now is 07:58 PM.


Powered by vBulletin® Version 3.8.9
Copyright ©2000 - 2017, vBulletin Solutions, Inc.
Single Sign On provided by vBSSO