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Custom recipe for GAII: one lane at a time mgravina Illumina/Solexa 0 03-27-2012 08:28 AM

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Old 04-18-2017, 09:28 AM   #1
r.rosati
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Default Chip Preparation Solution recipe?

Hello,
we have several Ion PI v2 chips, and we're successfully using them with Hi-Q chemistry kits. However, we're running short of Chip Preparation Solution, and Thermo has discontinued it. I know from the relevant patent that its composition should include a sulfonic acid in an organic solvent. I was wondering if the composition was ever disclosed by Ion Torrent in its "friendly" days or if anyone could suggest a possible recipe we could try.

Thank you!

EDIT: I think I got it, sorry. I just found a patent which describes a possible composition.

Last edited by r.rosati; 04-18-2017 at 09:57 AM.
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Old 04-18-2017, 11:09 PM   #2
avierstr
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I don't know if there is a lot of difference between the Proton and PGM chips, but I still use the 'old protocol' from in the beginning of the PGM.
- rinse the chip 2 times with 100 Ál isopropanol
- rinse the chip 3 times with annealing buffer
- perform chip check
- remove liquid
- load sample
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Old 04-20-2017, 07:21 AM   #3
r.rosati
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Thanks! I think I'll go with buying dodecyl benzene sulfonic acid (DBSA) and undecane, they're both relatively cheap; and prepare the 5% DBSA in undecane solution.
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