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Adapting Illumina's 16s library prep protocol to function with 18s rRNA giderk Sample Prep / Library Generation 4 04-23-2015 07:39 AM
Can I combine 16S and 18S rRNA in one sequencing run? cement_head Metagenomics 2 03-03-2015 06:10 AM

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Old 08-04-2020, 08:40 AM   #1
cement_head
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Location: Oxford, Ohio

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Question 16S & 18S Deep Sequencing Request(s)

Hello,

We've done 16S/18S/ITS/amplicon sequencing for years, with good results - sometimes excellent (library dependent) - for both internal and external clients.

We're starting to see more and more requests for guaranteed extremely high read depth per sample - like in the neighbourhood of 50,000 to 100,000 reads per side per PE sample. What kind of Illumina instrument (other than a MiSeq) does this (amplicon) sequencing well and why are people becoming obsessed with ultra-high read depth?

TIA
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Old 08-04-2020, 09:47 PM   #2
KevinLam
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Hmm e-dna?
environmental DNA?
deep metagenomics?

I think Ion Torrent does a fairly good job of this ..
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16s, amplicon sequencing, illumina, miseq, read depth

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