SEQanswers

Go Back   SEQanswers > Sequencing Technologies/Companies > Illumina/Solexa



Similar Threads
Thread Thread Starter Forum Replies Last Post
bowtie advantages over tophat frymor Bioinformatics 3 10-14-2011 07:23 AM
Need ideas about the advantages of Blast2Go byou678 Bioinformatics 5 08-27-2011 10:47 AM
Solexa: advantages/drawbacks/comparisions bioinf Illumina/Solexa 8 12-26-2010 02:41 PM
Advantages of third-generation rubenken The Pipeline 2 10-14-2010 01:48 AM
PubMed: Advantages of next-generation sequencing versus the microarray in epigenetic Newsbot! Literature Watch 0 06-19-2009 05:00 AM

Reply
 
Thread Tools
Old 10-06-2009, 06:30 PM   #1
seqgirl123
Member
 
Location: U.S

Join Date: Oct 2008
Posts: 76
Default advantages and disadvantages of adapter barcoding?

Hello,
What are the advantages and disadvantages of barcoding adapters for multiplexing versus barcoding the PCR primers? Is adapter barcoding just another way to do multiplex?
seqgirl123 is offline   Reply With Quote
Old 10-07-2009, 02:54 AM   #2
BENM
Member
 
Location: PRC

Join Date: May 2009
Posts: 33
Default

"Although indexing has the obvious benefit of multiplexing samples within a run, DNA indexing offers two key additional advantages: direct measure of base-by-base error rate and reduction of arrayto-array or day-to-day variability."

--by David WCraig etc. al., Identification of genetic variants using bar-coded multiplexed sequencing, Nature Methods, Vol. 5, No. 10. (14 September 2008), pp. 887-893.
BENM is offline   Reply With Quote
Reply

Thread Tools

Posting Rules
You may not post new threads
You may not post replies
You may not post attachments
You may not edit your posts

BB code is On
Smilies are On
[IMG] code is On
HTML code is Off




All times are GMT -8. The time now is 11:11 PM.


Powered by vBulletin® Version 3.8.9
Copyright ©2000 - 2020, vBulletin Solutions, Inc.
Single Sign On provided by vBSSO