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Old 02-17-2017, 01:22 PM   #1
Junior Member
Location: Indianapolis

Join Date: Feb 2017
Posts: 1
Default Low input RNA- Stranded sequencing

Hello all,

I am interested in knowing about stranded RNA library prep kits that I could use for low input RNA (as low as 3ng/uL) samples. I was looking at the following kits,

1. SMARTer stranded RNA-seq kit (minimum 100pg input RNA)
2. TruSeq stranded kit (minimum 100ng)
3. NEBNext Ultra RNA kit (minimum 10ng)
4. ScriptSeq V2 kit (minimum 0.5ng)
5. Nugene Ovation kit (minimum 0.5ng)
6. Kappabiosystems Hyperprep kit (unsure about the minimum required)

Other than TruSeq kit, I don't have any idea if any of the other kits are good. It would be great if someone that has used these kits could let me know if they were able to generate good quality data at low input amount.

It would be wonderful if any one could guide me through this.

Thank you very much!
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Old 02-20-2017, 07:06 AM   #2
MU Core
Location: Columbia, Missouri

Join Date: Apr 2008
Posts: 57

You may find this publication helpful.
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library prep, low input, rna sequencing, stranded rna-seq

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