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  • Help the rookie with how to use edgeR- please!

    Hi,
    I am completely new to any kind of bioinformatics and have just managed to get some raw counts from a sequencing project which were generated using Bowtie and Tophat.
    I would like to compare the sets of raw counts, which i have as a list text file with the Ensembl gene tag annotation and the count.
    I have managed to install R but i really need some help as I have no idea how to get the raw data into R and analyse it with edgeR! I have no experience with using html coding and no bioinformatics support available- i would be really grateful if someone out there could give me a dummy's guide to running the analysis!
    Thanks for your help.Finnola

  • #2
    Read the users guide first. It has a quick start:

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