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Old 03-21-2016, 05:47 AM   #1
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Location: United Kingdom

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Default rRNA removal for RNAseq analysis

Hi all,

Do you think we need to filter rRNA and low complexity reads from Poly-A selected total mRNAseq stranded data before performing RNAseq analysis (tophat2-htseq-deseq2)? I am sorry if I have missed any previous post on similar matter.

Thanks for your help.
genomica is offline   Reply With Quote
Old 03-21-2016, 12:31 PM   #2
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Location: California

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Generally not necessary, but it doesn't really hurt. Also, depending on what GTF file you provide to htseq, rRNAs may be excluded anyways.
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