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03-21-2016, 05:47 AM
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#1 |
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Junior Member
Location: United Kingdom Join Date: Feb 2013
Posts: 9
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rRNA removal for RNAseq analysis
Hi all,
Do you think we need to filter rRNA and low complexity reads from Poly-A selected total mRNAseq stranded data before performing RNAseq analysis (tophat2-htseq-deseq2)? I am sorry if I have missed any previous post on similar matter. Thanks for your help. |
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03-21-2016, 12:31 PM
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#2 |
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Senior Member
Location: California Join Date: Jul 2014
Posts: 198
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Generally not necessary, but it doesn't really hurt. Also, depending on what GTF file you provide to htseq, rRNAs may be excluded anyways.
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