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Hello everyone! Does someone have experience in sequencing amplicon libraries obtained from FFPE? I've heard that there are some specific Taq polimerase that can be used when amplifying FFPE (for example RESTORASE). I was wondering if these Taq are reliable and high-fidelity and if they can be used to prepare amplicon libraries to be sequenced on a 454 GS Junior platform. Does Anyone want to share some experience? Thanks
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The field of epigenetics has traditionally concentrated more on DNA and how changes like methylation and phosphorylation of histones impact gene expression and regulation. However, our increased understanding of RNA modifications and their importance in cellular processes has led to a rise in epitranscriptomics research. “Epitranscriptomics brings together the concepts of epigenetics and gene expression,” explained Adrien Leger, PhD, Principal Research Scientist on Modified Bases...Yesterday, 07:01 AM -
Proteins are often described as the workhorses of the cell, and identifying their sequences is key to understanding their role in biological processes and disease. Currently, the most common technique used to determine protein sequences is mass spectrometry. While still a valuable tool, mass spectrometry faces several limitations and requires a highly experienced scientist familiar with the equipment to operate it. Additionally, other proteomic methods, like affinity assays, are constrained...04-04-2024, 04:25 PM