Seqanswers Leaderboard Ad

Collapse

Announcement

Collapse
No announcement yet.
X
 
  • Filter
  • Time
  • Show
Clear All
new posts

  • Interpreation of RSEQC read_distribution.py results

    Dear Seqanswers List,

    Here is my ouput from read_distribution.py from RSEQC run from Galaxy

    Total Reads 54875279
    Total Tags 58170116
    Total Assigned Tags 54031503
    =====================================================================
    Group Total_bases Tag_count Tags/Kb
    CDS_Exons 39184887 10217883 260.76
    5'UTR_Exons 37214570 10963357 294.60
    3'UTR_Exons 58010731 4386101 75.61
    Introns 1539911688 6018229 3.91
    TSS_up_1kb 24518972 906468 36.97
    TSS_up_5kb 110212503 4956374 44.97
    TSS_up_10kb 201827137 13004223 64.43
    TES_down_1kb 27333233 59583 2.18
    TES_down_5kb 118264548 3689226 31.19
    TES_down_10kb 210002921 9441710 44.96

    My questions:
    Where does rRNA fit in this picture?
    What about other transcripts other than mRNA such as pseudogenes?

    Thanks and best wishes,
    Rich
    Richard Friedman,
    Columbia University

Latest Articles

Collapse

  • seqadmin
    Recent Advances in Sequencing Analysis Tools
    by seqadmin


    The sequencing world is rapidly changing due to declining costs, enhanced accuracies, and the advent of newer, cutting-edge instruments. Equally important to these developments are improvements in sequencing analysis, a process that converts vast amounts of raw data into a comprehensible and meaningful form. This complex task requires expertise and the right analysis tools. In this article, we highlight the progress and innovation in sequencing analysis by reviewing several of the...
    Yesterday, 07:48 AM
  • seqadmin
    Essential Discoveries and Tools in Epitranscriptomics
    by seqadmin




    The field of epigenetics has traditionally concentrated more on DNA and how changes like methylation and phosphorylation of histones impact gene expression and regulation. However, our increased understanding of RNA modifications and their importance in cellular processes has led to a rise in epitranscriptomics research. “Epitranscriptomics brings together the concepts of epigenetics and gene expression,” explained Adrien Leger, PhD, Principal Research Scientist...
    04-22-2024, 07:01 AM

ad_right_rmr

Collapse

News

Collapse

Topics Statistics Last Post
Started by seqadmin, Today, 06:57 AM
0 responses
7 views
0 likes
Last Post seqadmin  
Started by seqadmin, Yesterday, 07:17 AM
0 responses
13 views
0 likes
Last Post seqadmin  
Started by seqadmin, 05-02-2024, 08:06 AM
0 responses
19 views
0 likes
Last Post seqadmin  
Started by seqadmin, 04-30-2024, 12:17 PM
0 responses
21 views
0 likes
Last Post seqadmin  
Working...
X