Hi all,
I am a newbie in the field of transcriptomics analysis and I want to get more familiar with it. I know I should first normalize (or should there also be a transformation step) the results between experiments (we're doing a comparison study) and then map the reads against the refence. What I do not understand is what kind of statistical analysis should be used and what I need to look at after mapping. I know it's good to look at the "expression values" column, but could it also be wise to sometimes look at other columns?
Any input would be helpfull.
Cheers,
RickBioinf
Ps. We have a license for the CLC Genomics Workbench.
I am a newbie in the field of transcriptomics analysis and I want to get more familiar with it. I know I should first normalize (or should there also be a transformation step) the results between experiments (we're doing a comparison study) and then map the reads against the refence. What I do not understand is what kind of statistical analysis should be used and what I need to look at after mapping. I know it's good to look at the "expression values" column, but could it also be wise to sometimes look at other columns?
Any input would be helpfull.
Cheers,
RickBioinf
Ps. We have a license for the CLC Genomics Workbench.
Comment