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Old 08-30-2017, 03:05 AM   #1
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Location: Stirlingshire

Join Date: Sep 2015
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Default Illumina CHiP-Seq Kit storage problem

Hey folks,

I just took receipt of a brand new 48 library, Illumina CHiP-Seq kit. However, it had been initially delivered to our stores department, and they in their wisdom had decided to unpack the kit and deliver it to me at room temperature.

I reckon the whole kit (Box A & B) has been sitting at room temperature for a maximum of around 3hrs before I finally got it into the freezer. Should I be worried?

Craig xx
Beagleboy is offline   Reply With Quote
Old 09-14-2017, 05:28 AM   #2
Location: Montpellier (France)

Join Date: May 2008
Posts: 93


In my opinion, the most sensitive reagent in the kit is the ligation mix.
But it's hard to say if it suffered from standing at RT without testing it.
if you have any doubt, you can try to substitute it with NEB quick ligation kit or T4 DNA ligase.
huguesparri is offline   Reply With Quote

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