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Hi guys,
I have a feeling that everyone but me knows or has figured this out...
Why are the ends different in a RNA seq library? I can see that the both primers anneal in the 3' end to the adaptor sequence and that the genomic sequencing primer only anneal to the long PCR primer, but I can't figure out what prevents the final library to be a random selection of fragments with primer 1.1 or 2.1 in both ends and some with different ends. What am I missing?
Happy sequencing,
Darwin
I have a feeling that everyone but me knows or has figured this out...
Why are the ends different in a RNA seq library? I can see that the both primers anneal in the 3' end to the adaptor sequence and that the genomic sequencing primer only anneal to the long PCR primer, but I can't figure out what prevents the final library to be a random selection of fragments with primer 1.1 or 2.1 in both ends and some with different ends. What am I missing?
Happy sequencing,
Darwin
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