Hi, I have about 4 different MiSeq runs i have data for. I have pipelined the fastq files through bowtie-samtools-cuffdiff to compare cDNA libraries of different samples. A thought that just occured to me is some of the times i loaded a 15pM library and sometimes i loaded a 8pM library into the MiSeq cartridge (150bp v3) .
Does anyone know if its still possible to combine the data. Does the data get normalized against total number of reads?
Thanks, Will
Does anyone know if its still possible to combine the data. Does the data get normalized against total number of reads?
Thanks, Will
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