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  • dependent or independent samples for 16s samples of the same person at different time

    Hi,
    We did 2 16S microbiome sequencing experiments with 6 people in each.
    The 6 people are the same 6 people in both experiments.
    The only thing that have been changed is the time of the sequencing (not the time we took the sample).

    Time x for experiment 1, and time y for experiment 2.

    Can i treat a sequencing sample of person A in time x, and a sequencing sample of person A in time y as independent sample for statistics?

    Obviously, 2 sequencing of the same person at the same time on the same run are dependant, but in case of 16s microbiome with different time and different runs?

    Thanks,

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