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I am new to this forum, so I apologize if someone has asked this question already. I have been prepping total RNA from rat liver tissue using the miRNeasy kit from Qiagen and analyzing the RNA using the Agilent pico kit. My RIN values are averaging in the 2.3 range and I understand that the RIN value should be ~8. I was just wondering if others have used the same method of total RNA isolation from flash frozen tissue and what RIN values you typically see? Thanks for any help!
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Proteins are often described as the workhorses of the cell, and identifying their sequences is key to understanding their role in biological processes and disease. Currently, the most common technique used to determine protein sequences is mass spectrometry. While still a valuable tool, mass spectrometry faces several limitations and requires a highly experienced scientist familiar with the equipment to operate it. Additionally, other proteomic methods, like affinity assays, are constrained...04-04-2024, 04:25 PM -
Despite advancements in sequencing platforms and related sample preparation technologies, certain sample types continue to present significant challenges that can compromise sequencing results. Pedro Echave, Senior Manager of the Global Business Segment at Revvity, explained that the success of a sequencing experiment ultimately depends on the amount and integrity of the nucleic acid template (RNA or DNA) obtained from a sample. “The better the quality of the nucleic acid isolated...03-22-2024, 06:39 AM