Has anyone sucessfully sequenced 200 bp libraries originally constructed for Ion PGM, using Ion Proton.
We have been told by tech support that it was possible and should work well but our experience so far has been proven disastrous.
After some tests we know for a fact we don't have any instrument nor reagents problem. When we sequenced the same libraries in the PGM we got median lengths around 170 bp and in the PGM around 75 bp. The amount of reads with low quality and polyclonals is very big.
I am not convinced Proton chemistry can handle 200 bp PGM libraries.
We have been told by tech support that it was possible and should work well but our experience so far has been proven disastrous.
After some tests we know for a fact we don't have any instrument nor reagents problem. When we sequenced the same libraries in the PGM we got median lengths around 170 bp and in the PGM around 75 bp. The amount of reads with low quality and polyclonals is very big.
I am not convinced Proton chemistry can handle 200 bp PGM libraries.
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