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Old 10-02-2015, 06:14 AM   #1
AAR
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Location: MA

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Default Aptamer sequencing on MiSeq

I will like to sequence pools of aptamer sequences on a MiSeq. The aptamers are 100bp long. Are there any protocols avilable for this? What workflow would I follow when preparing the sample sheet on the Illumina?

Thanks!
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Old 10-08-2015, 08:41 PM   #2
BiochemPhD
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You may be interested in our FASTAptamer software. It was designed specifically for sequence analysis of combinatorial selections. Our user's guide has some information on workflow... that information is also in the publication.

Here's the post introducing the software on SEQanswers
http://seqanswers.com/forums/showthread.php?t=50754

Check out our publication:
Molecular Therapy Nucleic Acids (2015) 4, e230; doi:10.1038/mtna.2015.4

FASTAptamer software, sample data and a userís guide are available for download at http://burkelab.missouri.edu/fastaptamer.html

We also welcome development of our toolkit over on GitHub!

Feel free to reach out to us if you need assistance.
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