Dear All,
We are using Nextera Rapid Capture Custom Enrichment, after tagmentation, purification and first amplification, DNA concentrations are too low, not enough for 500ng suggested for hybridisation. Followed the protocol exactly. Any suggestion? How can I fix it?
Thanks all
Best
Andrea_G
We are using Nextera Rapid Capture Custom Enrichment, after tagmentation, purification and first amplification, DNA concentrations are too low, not enough for 500ng suggested for hybridisation. Followed the protocol exactly. Any suggestion? How can I fix it?
Thanks all
Best
Andrea_G