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Does anyone use the RiboZero kits for rRNA depletion of non-human/mouse/rat samples? I mean vero, BHK, or CHO etc. cells.
/Serge
/Serge
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Mammalian cultured cell lines should work well with the Ribo-Zero H/M/R kit. For other types of samples, please see our species compatibility list -
I recently used on lung RNA from cyno maqaques samples and it worked great.Comment
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I have problem with mouse.If you need some farm staff, check wytrzymałe sita do wialni petkus.Comment
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For specific organism depletion, have you considered using NuGEN’s model organism kits utilizing our InDA-C technology? For non-model organisms, we offer customization. The way this works is that you provide us with information on the organism of choice, and sequences which you want depleted (and this is not limited to ribosomal RNA, but other highly abundant transcripts) and we design specific probes to target these transcripts. For the more standard organism such as mouse and rat, we have off the shelf kits available:
DarrenComment
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The field of epigenetics has traditionally concentrated more on DNA and how changes like methylation and phosphorylation of histones impact gene expression and regulation. However, our increased understanding of RNA modifications and their importance in cellular processes has led to a rise in epitranscriptomics research. “Epitranscriptomics brings together the concepts of epigenetics and gene expression,” explained Adrien Leger, PhD, Principal Research Scientist...Yesterday, 07:01 AM -
Proteins are often described as the workhorses of the cell, and identifying their sequences is key to understanding their role in biological processes and disease. Currently, the most common technique used to determine protein sequences is mass spectrometry. While still a valuable tool, mass spectrometry faces several limitations and requires a highly experienced scientist familiar with the equipment to operate it. Additionally, other proteomic methods, like affinity assays, are constrained...04-04-2024, 04:25 PM
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