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Introducing mothur: Open Source, Platform-independent, Community-supported Software for Describing and Comparing Microbial Communities.
Appl Environ Microbiol. 2009 Oct 2;
Authors: Schloss PD, Westcott SL, Ryabin T, Hall JR, Hartmann M, Hollister EB, Lesniewski RA, Oakley BB, Parks DH, Robinson CJ, Sahl JW, Stres B, Thallinger GG, Van Horn DJ, Weber CF
mothur aims to be a comprehensive software package that allows users to use a single piece of software to analyze community sequence data. It builds upon previous tools to provide a flexible and powerful software package for analyzing sequencing data. As a case study, we used mothur to trim, screen, and align sequences, calculate distances, assign sequences to OTUs, and describe the alpha- and beta-diversity of eight marine samples previously characterized by pyrosequencing of 16S rRNA gene fragments. This analysis of more than 222,000 sequences was completed in less than 2 hours using a laptop computer.
PMID: 19801464 [PubMed - as supplied by publisher]
More...
Introducing mothur: Open Source, Platform-independent, Community-supported Software for Describing and Comparing Microbial Communities.
Appl Environ Microbiol. 2009 Oct 2;
Authors: Schloss PD, Westcott SL, Ryabin T, Hall JR, Hartmann M, Hollister EB, Lesniewski RA, Oakley BB, Parks DH, Robinson CJ, Sahl JW, Stres B, Thallinger GG, Van Horn DJ, Weber CF
mothur aims to be a comprehensive software package that allows users to use a single piece of software to analyze community sequence data. It builds upon previous tools to provide a flexible and powerful software package for analyzing sequencing data. As a case study, we used mothur to trim, screen, and align sequences, calculate distances, assign sequences to OTUs, and describe the alpha- and beta-diversity of eight marine samples previously characterized by pyrosequencing of 16S rRNA gene fragments. This analysis of more than 222,000 sequences was completed in less than 2 hours using a laptop computer.
PMID: 19801464 [PubMed - as supplied by publisher]
More...