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I have used Primer3 tool within Geneious to design a large number of primers. There is one parameter that I would like some help in interpreting. The primer dimer score, which ranges from 0 to a large number of some kind, is somewhat confusing. How is this calculated? What is considered a “good” number for primer dimers not to form? Obviously zero is ideal, but when I look at my dataset, It ranges up to 30 or so, with everything in between. The online help information gives examples of 6 or 7. I would appreciate any laboratory experience advice on what would be a good threshold to avoid. I am developing primers for Fluidigm/Illumina. Thanks!
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The field of epigenetics has traditionally concentrated more on DNA and how changes like methylation and phosphorylation of histones impact gene expression and regulation. However, our increased understanding of RNA modifications and their importance in cellular processes has led to a rise in epitranscriptomics research. “Epitranscriptomics brings together the concepts of epigenetics and gene expression,” explained Adrien Leger, PhD, Principal Research Scientist on Modified Bases...Yesterday, 07:01 AM -
Proteins are often described as the workhorses of the cell, and identifying their sequences is key to understanding their role in biological processes and disease. Currently, the most common technique used to determine protein sequences is mass spectrometry. While still a valuable tool, mass spectrometry faces several limitations and requires a highly experienced scientist familiar with the equipment to operate it. Additionally, other proteomic methods, like affinity assays, are constrained...04-04-2024, 04:25 PM