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Hello, I have very difficult time with enrichment. Most libraries need high copies per bead to give >5% enrichment rate. I use 10 copies or more. This is strange to me. I usually use much less than 10 copies per bead. I am afraid this will cause mixed Signals. How come I cant get good values anymore? Anyone have this problem? Thank you for help.
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The field of epigenetics has traditionally concentrated more on DNA and how changes like methylation and phosphorylation of histones impact gene expression and regulation. However, our increased understanding of RNA modifications and their importance in cellular processes has led to a rise in epitranscriptomics research. “Epitranscriptomics brings together the concepts of epigenetics and gene expression,” explained Adrien Leger, PhD, Principal Research Scientist...04-22-2024, 07:01 AM -
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