hello,
I encountered some weird thing after my library amplification with classical illumina library prep.
I went through size selection with sybrgold agarose gel, did analysis on Agilent Bioanalyzer, and got expected peaks.
After that I did standart illumina library amplification (10cycles), did again Agilent analysis and beside expected peak I got some weird peak in region of ˜3kb.
Has anyone any ideas? Is it some kind of chimeric entities emerged during PCR? This was E.coli gDNA.
Thanks!
I encountered some weird thing after my library amplification with classical illumina library prep.
I went through size selection with sybrgold agarose gel, did analysis on Agilent Bioanalyzer, and got expected peaks.
After that I did standart illumina library amplification (10cycles), did again Agilent analysis and beside expected peak I got some weird peak in region of ˜3kb.
Has anyone any ideas? Is it some kind of chimeric entities emerged during PCR? This was E.coli gDNA.
Thanks!