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  • cuffdiff problems: only got 3 records in the gene_exp.diff file

    Hi,
    I set up a cuffdiff running. Here is my code:
    cuffdiff -o /home/twotwo /home/twotwo/reference.gtf {samples from group1 separated by comma}{space}{samples from group2 separated by comma}

    Then the log looks fine for the first hundreds lines, with some loci processing...
    Here is the last part:

    Performed 188097 isoform-level transcription difference tests
    Performed 0 tss-level transcription difference tests
    Performed 3 gene-level transcription difference tests
    Performed 0 CDS-level transcription difference tests
    Performed 0 splicing tests
    Performed 0 promoter preference tests
    Performing 0 relative CDS output tests
    Writing isoform-level FPKM tracking
    Writing TSS group-level FPKM tracking
    Writing gene-level FPKM tracking
    Writing CDS-level FPKM tracking
    Writing isoform-level count tracking
    Writing TSS group-level count tracking
    Writing gene-level count tracking
    Writing CDS-level count tracking
    Writing isoform-level read group tracking
    Writing TSS group-level read group tracking
    Writing gene-level read group tracking
    Writing CDS-level read group tracking
    Writing read group info
    Writing run info


    The problem is: There should be 188097 probes for comparing. However, I only got 3 probes (3 rows) in the gene expression output. Any comments on that? very confused.

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