Hi,
I have a question regarding the RNASeq.
I am working with Human cell lines. For RNA Seq :
i) Should I consider Paired end sequencing or Single end?
ii) I am planning to get the sequencing done on Illumina for 6 samples in duplicates. Is having replicates for each samples a must? In my case if I consider duplicates then I will have 12 samples all together.
iii) Will it good to pool the 12 samples across 4 lanes in Illumina sequencer? My aim to ask this question is that how much depth are we looking at in case of RNA Seq?
Thank you for your kind attention.
I have a question regarding the RNASeq.
I am working with Human cell lines. For RNA Seq :
i) Should I consider Paired end sequencing or Single end?
ii) I am planning to get the sequencing done on Illumina for 6 samples in duplicates. Is having replicates for each samples a must? In my case if I consider duplicates then I will have 12 samples all together.
iii) Will it good to pool the 12 samples across 4 lanes in Illumina sequencer? My aim to ask this question is that how much depth are we looking at in case of RNA Seq?
Thank you for your kind attention.
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