Go Back   SEQanswers > Applications Forums > Sample Prep / Library Generation

Similar Threads
Thread Thread Starter Forum Replies Last Post
TruSeq or Nextera kit for library prep? rooty Sample Prep / Library Generation 12 10-29-2013 06:02 AM
Can Truseq DNA library get >1ug DNA library at last? rnal Illumina/Solexa 2 04-12-2012 07:36 AM
Truseq Small RNA kit - losing library in the final cleanup? Tali7 Sample Prep / Library Generation 3 10-12-2011 03:26 AM
Making the TruSeq RNA-sample prep kit go further? James Sample Prep / Library Generation 3 06-20-2011 10:46 AM
Which DNA Pol is equivalent to TruSeq kit? jlove Illumina/Solexa 4 04-29-2011 10:42 AM

Thread Tools
Old 09-28-2011, 04:09 PM   #1
Location: US

Join Date: Mar 2011
Posts: 20
Default Truseq RNA kit for making DNA library

I want to make 500bp paired-end library from genomic DNA. I already have the Truseq RNA kit. I only have two samples and don't want to order the expensive Truseq DNA kit. I check the manual and wandering maybe we can use the RNA kit from end repair step after sonicating. Does anyone try this?

Thanks a lot!
elisadouzi is offline   Reply With Quote
Old 05-07-2013, 02:19 AM   #2
Junior Member
Location: Vienna

Join Date: May 2013
Posts: 1

Although your post is from long ago, I was wondering how you finally ended up. I am in a similar situation now.
I read that the adaptors in the RNA and DNA TruSeq kit are the same, though the concentrations are not.
Zahra-A is offline   Reply With Quote
Old 05-22-2013, 11:58 AM   #3
Location: NYS

Join Date: Feb 2013
Posts: 15

It works totally fine. I've done it. The two kits a indistinguishable once you have clean dsDNA. The primers and reaction mixes are at the same concentrations.
Manna is offline   Reply With Quote
Old 07-17-2013, 07:32 AM   #4
Junior Member
Location: Taipei, Taiwan

Join Date: Jul 2013
Posts: 3

We did use RNA kit for all of our RNA/DNA cases. They were doing well and we could happily maximize the use of the RNA kit.
Riva is offline   Reply With Quote

Thread Tools

Posting Rules
You may not post new threads
You may not post replies
You may not post attachments
You may not edit your posts

BB code is On
Smilies are On
[IMG] code is On
HTML code is Off

All times are GMT -8. The time now is 11:26 PM.

Powered by vBulletin® Version 3.8.9
Copyright ©2000 - 2020, vBulletin Solutions, Inc.
Single Sign On provided by vBSSO