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Old 09-28-2011, 04:09 PM   #1
elisadouzi
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Location: US

Join Date: Mar 2011
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Default Truseq RNA kit for making DNA library

I want to make 500bp paired-end library from genomic DNA. I already have the Truseq RNA kit. I only have two samples and don't want to order the expensive Truseq DNA kit. I check the manual and wandering maybe we can use the RNA kit from end repair step after sonicating. Does anyone try this?

Thanks a lot!
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Old 05-07-2013, 02:19 AM   #2
Zahra-A
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Question

Although your post is from long ago, I was wondering how you finally ended up. I am in a similar situation now.
I read that the adaptors in the RNA and DNA TruSeq kit are the same, though the concentrations are not.
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Old 05-22-2013, 11:58 AM   #3
Manna
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It works totally fine. I've done it. The two kits a indistinguishable once you have clean dsDNA. The primers and reaction mixes are at the same concentrations.
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Old 07-17-2013, 07:32 AM   #4
Riva
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We did use RNA kit for all of our RNA/DNA cases. They were doing well and we could happily maximize the use of the RNA kit.
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