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Old 03-27-2012, 07:19 PM   #1
eosin
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Default MiSeq Bacterial Genome Multiplexing

Can I multiplex bacterial genomes on MiSeq? If so, how many ? Thank you!
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Old 03-27-2012, 09:54 PM   #2
ECO
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Uh...how much coverage will be useful for you?

Today = 8-9M PF read pairs (2x150bp from each) = ~2.6GB = ~566x coverage of one E.coli sized genome (4.5MB). Divide at will.
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Old 03-27-2012, 11:29 PM   #3
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What does PF stand for? Sorry, I'm a newbie in NGS.
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Old 03-28-2012, 03:23 AM   #4
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Quote:
Originally Posted by eosin View Post
What does PF stand for? Sorry, I'm a newbie in NGS.
It means Pass Filter - not all reads generated pass the filtering process eg a run could generate 10 million reads but only 9 million are passed by the quality filtering process.
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Old 03-28-2012, 05:02 PM   #5
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Quote:
Originally Posted by protist View Post
It means Pass Filter - not all reads generated pass the filtering process eg a run could generate 10 million reads but only 9 million are passed by the quality filtering process.
Thank you!
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