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Old 05-29-2012, 02:01 PM   #1
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Default Cufflinks models from gsnap alignments extend beyond the reference

I'm using Cufflinks 2.0.0 without a reference GTF to build models from GSNAP v20120427 alignments, also made without a reference GTF (i.e. using -N 1). I noticed that some of the Cufflinks models extend beyond the end of the reference contig sequence. They also seem to extend beyond the end of the GSNAP alignments as shown in a pileup file.

For example, cufflinks reported this transcript:
scaffold02137	Cufflinks	exon	4297	4727	1000	-	.	gene_id "CUFF.17382"; transcript_id "CUFF.17382.1"; exon_number "2"; FPKM "95.5022086611"; frac "1.000000"; conf_lo "86.719816"; conf_hi "104.284601"; cov "139.246274";
But scaffold01237 is only 4696 bases long, and according to the pileup, GSNAP's alignment ends at base 4696.

Is this a known problem with this combination of Cufflinks 2.0.0 and GSNAP 20120427? I can't see how Cufflinks would know to extend the transcript if there isn't data in GSNAP's SAM/BAM output to begin with, and it makes me wonder if Cufflinks is parsing the BAM file incorrectly.
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