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Old 08-28-2012, 07:44 AM   #1
madseq
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Default KAPA qPCR kit for XL+ libraries?

Are you successfully quantifying your XL+ libraries with this kit? Have you modified (extended) cycling times?

I have tried with extended denaturation and extension times and dindīt get very good results...

Any help?
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Old 08-28-2012, 04:24 PM   #2
RCJK
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Hi there,

Yes I do this. I use a 2 minute extension time and it seems pretty consistent. Good luck!
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Old 08-31-2012, 07:39 AM   #3
madseq
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Hi RCJK.
I tried with 30secs denaturation time and 2 min annealing/extension time and it worked just fine. Thank you!
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