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Old 01-07-2013, 09:33 AM   #1
ChIP Kick
Junior Member
Location: Phoenix

Join Date: Dec 2012
Posts: 4
Default Why there are blobs in my sheared DNA bands?

Hi, I have some problems with my sheared DNA now. Hope you can help me! Attached are two images for the same DNA gel. Image were taken at different time. One is at 1:00 pm and another is at 3:11pm.

It seems like that I get some smeared DNA. But I do not know why there are blobs at the bottom of the band. I treated samples with proteinase K, incubate at 58 degree Celsius, overnight. Then did RNase A treatment at 37degree Celsius for 1 hour. After that, run a agarose gel at 53 voltage. Most sheared DNA bands in web don't have blobs. Could you give me some suggestions about that?

Thanks a lot!
Attached Images
File Type: jpg 1pm gel.jpg (20.6 KB, 22 views)
File Type: jpg 3pm gel.jpg (19.9 KB, 15 views)
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rnase a treatment, sheared dna

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