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Old 07-12-2013, 11:18 AM   #1
slambert
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Location: Canada

Join Date: Jul 2013
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Default C.elegans rRNA reduction kits

I'm wondering if anyone out there has tried/compared multiple rRNA subtraction methods of C. elegans total RNA or has any comments on the matter?

I'd like to use a kit for RNA isolation that doesn't involve poly-A selection so as not to deplete my sample of possibly un-polyadenylated RNAs. I know that there are 2 major kits out there Ribo-Zero(epicenter/Illumina) and Ribo-Minus (Invitrogen) and was hoping that some of you might know which one works better for reducing C. elegans rRNA. Any info is greatly appreciated!
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Old 09-30-2013, 03:47 PM   #2
epistatic
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Epicentre's Ribo-zero magnetic kit and 100 ng of RNA = 0.2% ribosomal bases
Epicentre's Ribo-zero magnetic kit and 1 ug of RNA = 30% ribosomal bases
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c.elegans, ribo-zero, ribominus, rna, rrna removal

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