Go Back   SEQanswers > Sequencing Technologies/Companies > Illumina/Solexa

Similar Threads
Thread Thread Starter Forum Replies Last Post
Selections of library preparation based on panel for MiniSeq namtran93 Illumina/Solexa 1 06-16-2017 09:04 AM
Library Prepration for MiniSeq Help valenex Illumina/Solexa 8 03-08-2017 05:53 AM
Custom primers on a MiniSeq? jfostel Illumina/Solexa 1 07-20-2016 11:36 AM
MiniSeq - a new addition? GenoMax Illumina/Solexa 31 01-27-2016 08:47 AM
First info on the miniSeq cibertech The Pipeline 16 01-11-2016 09:18 AM

Thread Tools
Old 06-10-2018, 11:17 PM   #1
Junior Member
Location: Ho Chi Minh city, Vietnam

Join Date: May 2017
Posts: 9
Default Undercluster on MiniSeq system

Hello everyone,

As the title, I ran a sequencing reaction last week on MiniSeq system by Mid-Output kit 300 cycles. I determined the concentrations of library by BioAnalyzer HS chip and diluted the pooled library to 1.2 pM. When the reaction completed, the system notified me that it was undercluster (only 141k/mm2). Two points make me confused that: (1) I have optimized the concentration of library to 1.2 pM already and it was good for other runs;(2) Illumina said that the library should be diluted to 1.1 pM if I use BioAnlyzer as a quantification methods.

I hope that someone met the similar problem with me and give me some suggestions.
Nam Tran-huynh-bao
namtran93 is offline   Reply With Quote

Thread Tools

Posting Rules
You may not post new threads
You may not post replies
You may not post attachments
You may not edit your posts

BB code is On
Smilies are On
[IMG] code is On
HTML code is Off

All times are GMT -8. The time now is 01:24 PM.

Powered by vBulletin® Version 3.8.9
Copyright ©2000 - 2020, vBulletin Solutions, Inc.
Single Sign On provided by vBSSO