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  • how to evaluate raw data

    Hey, i'm a layman in Bioinformatics. Our lab have completed a project of mRNA-sequencing on illumina platform and obtained about 30 millions 150bp-200bp reads. The sample is a mouse cell line.
    i have some questions. for example,how to evaluate the raw data? is there any way to check the qulity of raw data, good or bad?
    thank you for help. Best Wishes.

  • #2
    you might try fastQC which does a quality control on raw illumina data;

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